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The Cell and Tissue Imaging Core facility operates under the direction of Dr. David Hinton and is supervised by Ernesto Barron and Anthony Rodriguez. It is located in a 2,100-square-foot, shared-use facility on the USC Health Science Campus within the Doheny Vision Research Center (DVRC) Room 214. The facility is available to all in the USC community by appointment 24 hours/day. Training and assistance in the use of the instruments and associated technical procedures is provided by appointment and in scheduled in-service training sessions.
The primary function of the Cell and Tissue Imaging Core Facility is to provide assistance and training in the operation of equipment for laser scanning confocal microscopy, multiphoton laser scanning confocal microscopy (Zeiss LSM510), Spinning disk confocal microscopy (PerkinElmer Ultraviewer pinning disk confocal),transmission electron microscopy (JEOL JEM 2100 LaB6), scanning electron microscopy (JEOL JSM/6390LV), Laser Capture Microdissection Microscopy (Zeiss PALM System), fluorescence microscopy, quantitation of immunocytochemical procedures (Leica and Olympus Microscopes), and fluorescence and brightfield microdissection and microcapture (Zeiss PALM System). The Core facility offers support for specimen preparation, thin sectioning techniques, embedding procedures, cryosectioning techniques, photography, digital photomicroscopy and photomicrography and computer aided graphics.
Current services offered by the cell and tissue core module include specimen preparation and training in the operation of laser scanning confocal microscopy, multiphoton laser scanning confocal microscopy, Spinning disk confocal microscopy (live cell imaging), transmission electron microscopy (TEM), scanning electron microscopy (SEM), fluorescence microscopy and quantitation, of immunocytochemical procedures, fluorescence and brightfield microdissection and microcapture, thin sectioning techniques, embedding procedures, cryosectioning techniques, photography, digital photomicroscopy and photomicrography and computer aided graphics.
Confocal Service Request Form on website*
SEM turn around time is 1 week.
TEM turn around time is 2 weeks.
The Cell and Tissue Imaging Core Facility under the direction of David R. Hinton MD, and the supervision of Mr. Ernesto Barron, provides support for research projects requiring specialized microscopic imaging. The facility is available to all in the USC community by appointment 24 hours/day. Training and assistance in the use all of the instruments and associated technical procedures is provided by appointment and in scheduled in-service training sessions.
The primary function of the Cell and Tissue Imaging Core Facility is to provide assistance and training in the operation of equipment for laser scanning confocal microscopy, multiphoton laser scanning confocal microscopy (Zeiss LSM510), Spinning disk confocal microscopy (PerkinElmer Ultraviewer pinning disk confocal),transmission electron microscopy (JEOL JEM 2100 LaB6), scanning electron microscopy (JEOL JSM/6390LV), Laser Capture Microdissection Microscopy (Zeiss PALM System), fluorescence microscopy, quantitation of immunocytochemical procedures (Leica and Olympus Microscopes), and fluorescence and brightfield microdissection and microcapture (Zeiss PALM System). The Core facility offers support for specimen preparation, thin sectioning techniques, embedding procedures, cryosectioning techniques, photography, digital photomicroscopy and photomicrography and computer aided graphics.
USC/Norris Comprehensive Cancer Center
Cell and Tissue Imaging Core
Doheny Eye Institute
DVRC rm 214
1355 San Pablo St.
Los Angeles, CA 90089
Email: CoreConfocal@ccnt.usc.edu
Dr. David R. Hinton, M.D.
Director
Department: Pathology
Division: Keck School of Medicine
Health Sciences Campus DOH-213
Telephone: (323) 442-6617
Fax: (323) 442-6688
E-mail: dhinton@usc.edu
Ernesto Barron
Core Manager
Department: Pathology
Division: Keck School of Medicine
Health Sciences Campus DVCR 214
Telephone: 323 442-6622
Fax: 323 442-6688
E-mail: barron@usc.edu
Anthony Rodriguez
Core Technician
Department: USC Cancer Center
Division: Keck School of Medicine
Health Sciences Campus DEH 214
Telephone: (323)442-6622
Fax: 323 442-6688
Email: anrodrig@usc.edu
Scheduling appointments can be done in person at the core facility, via phone by calling or by emailing Ernesto Barron.