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The USC School of Pharmacy Translational Research Laboratory, located on the Jane and Gale Bensussen Research Floor (5th floor) of the Pharmaceutical Sciences Center, is designed to promote translational therapeutic discovery. This school-wide core laboratory is composed of two major units. The Bioinformatics/Computational Chemistry Unit is equipped with graphic workstations and modeling programs that provide for computer-based virtual screening and rational drug design applications. The Therapeutic Screening Unit houses a number of state-of-the-art specialized instruments that enable a broad range of automated and multiplexed biological analyses in a throughput manner. The primary mission of the Translational Research Laboratory is to provide USC researchers with full and timely access to lab resources and technical guidance. Moreover, the lab is available as a platform to promote translational research and provide a learning environment for new technologies and applications.
ABI 7900HT Fast Real-Time PCR System, equipped with Sequence Detection System 2.3 and RQ Manager 1.2, allows for fast and sensitive high-throughput gene expression profiling in a 96- or 384-well plate format. The Microfluidic Card Upgrade enables Taqman Low-Density Array (TLDA) analysis of 1 to 8 samples against 12 to 384 genes in a single run with a single addition of each sample.
BD Pathway 435 High-Content Bioimager, equipped with BD AttoVision 1.6, allows for automated capture of microscopic images from a 96-well plate and automated image and data analysis. An additional module allows for analysis of neurite outgrowth in a high-throughput manner.
BioRad Bio-Plex Suspension Array System, powered by the Luminex xMAP technology, includes Bio-Plex 200 System with HTF and Bio-Plex Pro II Wash Station. The system, equipped with Bio-Plex Manager 5.0, allows for magnetic or polystyrene bead-based multiplex bioassay that enables the simultaneous analysis of up to 100 biomolecules (proteins, peptides or nucleic acids) in a single sample.
BioRad Experion Automated Electrophoresis System that includes Automated Electrophoresis Station, Priming Station and Vortex Station, enables microfluidic chips-based eletrophoresis for analysis of DNA, RNA and protein size, quantity and quality.
BioRad Gene Pulser MXcell Electroporation System that includes a power module and a plate chamber, allows for the plate-based electroporation of mammalian cells - especially primary and hard-to-transfect cell types.
BioRad Gene Pulser Xcell Electroporation System that includes a main unit, two accessory modules (CE and PC modules), a ShockPod cuvette chamber, allows for the cuvette-based electroporation of mammalian, bacteria and fungal cell lines.
Horiba Jobin Yvon Fluorolog-3 Spectrofluorometer, equipped with computer-controlled excitation wavelength, emission wavelength, temperature, and polarization, allows for both steady-state and time-resolved characterization of fluorescent samples.
Molecular Devices Lmax Microplate Luminometer with Injectors, equipped with SOFTmax Pro for Lmax, allows for a variety of luminescence-based applications.
Molecular Devices SpectraMax Gemini EM Microplate Spectrofluorometer, equipped with SOFTmax Pro, allows for a variety of fluorescence-based applications. Main features include top and bottom reading optics for both solution and cell-based assays, dual monochromators enabling selection of any wavelength in 1 nm increments, wavelength scanning, well scanning and auto PMT gain.
Seahorse XF24 Extracellular Flux Analyzer allows for simultaneous measurement of two major energy-yielding pathways – anaerobic glycolysis and aerobic respiration in cell cultures or isolated mitochondria in a 24-well plate format.
SGI Octane Graphical Workstation equipped with the IRIX 6.5 Operating System and a comprehensive suite of computer modeling and simulation program including Accelrys InsightII 2000, allows for in silico virtual screening and structure-based rational drug design applications.
Tecan GENios Pro Multidection Microplate Reader with Injectors, equipped with XFluor4GENiosPro, allows for a variety of multidetection including fluorescence intensity, time-resolved fluorescence, fluorescence polarization, absorbance and luminescence-based applications.
Wyatt DynaPro Plate Reader uses laser light scattering to determine the hydrodynamic radius of particles, polymers, or proteins in solution in the size range of 2-1000 nm in diameter. It can be set up to do kinetic and temperature based assays in a 96 or 384-well plate in volumes down to 10 uL per sample.
| Instruments | Charge/Hour | Charge/Year with Unlimited Access | ||
| School of Pharmacy | Other USC Users | School of Pharmacy | Other USC Users | |
| Seahorse XF24 Extracellular Flux Analyzer(L) | $20 | $24 | $2,000 | $2,400 |
| ABI 7900HT Fast Real-Time PCR System (L) | $15 | $18 | $1,500 | $1,800 |
| BioRad Bio-Plex Suspension Array System (L) | $15 | $18 | $1,500 | $1,800 |
| BD Pathway 435 High-Content Bioimager (L) | $10 | $12 | $1,000 | $1,200 |
| Wyatt DynaPro Plate Reader | $7.5 | $9 | $750 | $900 |
| Horiba Jobin Yvon Fluorolog-3 Spectrofluorometer | $5 | $6 | $500 | $600 |
| Bio-Rad Experion Automated Electrophoresis System (S) | N/A | $200 | $240 | |
| Bio-Rad Gene Pulser MXcell Electroporation System (S) | N/A | $200 | $240 | |
| Bio-Rad Gene Pulser Xcell Electroporation System (S) | N/A | $200 | $240 | |
| Prospect Edge 200 Separation System (S) | N/A | $200 | $240 | |
| MD SpectraMax Gemini EM Microplate Spectrofluorometer (S) | N/A | $200 | $240 | |
| MD Lmax Microplate Luminometer (S) | N/A | $200 | $240 | |
| Tecan GENios Pro Multidetection Microplate Reader (S) | N/A | $200 | $240 | |
| SGI Octane Graphical Workstation | Discuss with lab manage | |||
Technical training and assistance with applications will be provided by lab technical personnel. Charges will be based on hours/applications or yearly unlimited usage.
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The Translational Research Laboratory in the USC School of Pharmacy provides access to a number of state-of-the-art instruments for translational therapeutic discovery and development research. The Bioinformatics and Computational Chemistry Unit provides for computer modeling-based virtual screening and rational drug design applications. The Therapeutic Screening Unit provides for a broad range of automated and multiplexed from gene to whole cell biological analyses in a medium to high-throughput manner. Instruments housed in the lab include: Seahorse XF24 Extracellular Flux Analyzer, ABI 7900HT Fast Real-Time PCR System, BioRad Bio-Plex Suspension Array System, BD Pathway 435 High-Content Bioimager, BioRad Experion Automated Electrophoresis System, BioRad Gene Pulser MXcell Electroporation System, BioRad Gene Pulser Xcell Electroporation System, Wyatt DynaPro Plate Reader, Horiba Jobin Yvon Fluorolog-3 Spectrofluorometer, Molecular Devices SpectraMax Gemini EM Microplate Spectrofluorometer, Molecular Devices Lmax Microplate Luminometer, Tecan GENios Pro Multidetection Microplate Reader, and SGI Octane Graphical Workstation equipped with computer modeling programs.
Pharmaceutical Sciences Center, Room 510 Health Science Campus USC School of Pharmacy 1985 Zonal Avenue Los Angeles, CA 90033
For lab resources and appointments:
Liqin Zhao, PhD
Research Assistant Professor Pharmacology and Pharmaceutical Sciences
Manager – Translational Research Laboratory
USC School of Pharmacy PSC, Room 508
Telephone: (323) 442-1495
E-mail: liqinz@usc.edu
For administrative and other general issues:
Sarah F. Hamm-Alvarez, PhD
Gavin S. Herbert Professor and Chair
Department of Pharmacology and Pharmaceutical Sciences
Associate Dean for Research Affairs
USC School of Pharmacy PSC, Room 704
Telephone: (323) 442-1445
E-mail: shalvar@pharmacy.usc.edu
Joanne T. Lee
Budget/Business Analyst
Office of the Associate Dean for Research Affairs
USC School of Pharmacy PSC – 100
Telephone: (323) 442-2612
E-mail: leejt@usc.edu
For inquiries, please contact:
Liqin Zhao, Ph.D.
Lab Manager
Telephone: (323) 442-1495
E-mail: liqinz@usc.edu