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The USC/Norris Comprehensive Cancer Center Transgenic Core Facility. The Facility was established in 1993 to provide high-quality embryo and stem cell manipulation, microinjection and related services to the USC community, as well as outside researchers. The core handles the production of transgenic mice, gene targeting and the production of knockout Mice, Construction targeting vector design, preparation of DNA for injection and In vitro fertilization, Re-derivation, as well as Cryopreservation. The Facility is located in both Norris Cancer Center and Zilkha Neurogenetic Institute of USC Medical Center. It comprises a laboratory for DNA purification and quantitation; a cell culture room for performance of ES cell culture, electroporation, pick up clones; a microinjection room for isolation, microinjection and implantation of the fertilized mouse embryos, as well as adjoining vivaria.
TRANSGENIC MOUSE Orders- We will pay for the maintenance of foster mothers and pups up to three weeks from date of birth. During that time we expect the PI to (1) Genotype the mice; (2) Transfer the mice to the PI's vivarium protocol. We will charge double for the mice left on the Core protocol after three weeks. In general, the injection will be repeated until at least 2 transgenic pups are obtained (except the cases in which the transgenic causes embryonic lethality). Transgenic status refers only to DNA integration as assesed by Southern blot. We can't guarantee expression of an RNA or protein product from the integrated DNA.
Please contact us for more information.
USC/Norris Comprehensive Cancer Center
Transgenic Core Facility
1441 Eastlake Ave. Room 5318
Los Angeles, CA 90089-9176
Phone: 323/865-0726
Fax: 323/865-0098
Email: CoreTransgenic@ccnt.usc.edu
Robert Maxson
Director
Department: Biochemistry and Molecular Biology
Division: Keck School of Medicine
Campus: Health Sciences Campus
Building Code: NOR 5334
Mail Code: 9176
Telephone: (323) 865-0633
Fax: (323) 865-0098
Email: maxson@usc.edu
Nancy Lanying Wu, M.D.
Transgenic/Knockout Services Manager
Department: USC Cancer Center
Division: Keck School of Medicine
Campus: Health Sciences Campus
Building Code: NOR 7310
Mail Code: 9176
Telephone: (323) 865-0726
Fax (323) 865-0098
Email: nwu@usc.edu
Youzhen Yan
Injection Technician:
Department: USC Cancer Center
Division: Keck School of Medicine
Campus: Health Sciences Campus
Building Code: NOR 7310
Mail Code: 9176
Telephone: (323) 865-0639
Fax: (323) 865-0098
Email: youzhen@usc.edu
John Johnson
Financial Assistant
Department: USC Cancer Center
Division: Keck School of Medicine
Campus: Health Sciences Campus
Building Code: NOR 5334
Mail Code: 9176
Telephone: (323) 865-0639
Fax: (323) 865-0098
Email: john.e.johnson@usc.edu
Linda Liu, Ph.D.
Gene Targeting Manager
Department: USC Cancer Center
Division: Keck School of Medicine
Campus: Health Sciences Campus
Building Code: NOR 5334
Mail Code: 9176
Telephone: (323) 865-0635
Fax: (323) 865-0098
Email: liqiongl@usc.edu
Contact us for further information.