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The USC School of Pharmacy Histology Core Laboratory, located on the 4th floor of the Pharmaceutical Sciences Center, is ready to help you with your histology and pathology research. This school-wide core laboratory is open to any USC researcher and has everything you will need to make microscope slides from tissue samples. Tissues can be paraffin embedded, sectioned and stained in the laboratory. Sectioning can involve making microtome, vibratome and cryostat thin slices. You can also make color photographs of your microscopic images for publication. Expert advice is available concerning staining techniques and the interpretation of microscopic images.
The Histology Laboratory houses the following instruments and accessories:
Components/Accessories:
Capacity:The tissue processor automatically dehydrates and paraffin infiltrates tissue samples.
Components/Accessories:
Capacity: At this station, paraffin infiltrated tissues are embedded in wax and mounted on cassettes.
Components/Accessories:
Capacity: The microtome cuts thin sections of paraffin embedded tissue cassettes with a disposable blade. The blade can be quickly replaced as necessary. The water bath is used to float the thin sections onto microscope slides.
Components/Accessories:
Capacity: The vibratome is used to cut sections of formaldehyde fixed soft tissues, such as brain. Sections are mounted onto microscope slides.
Capacity: Frozen tissue samples are thin sectioned with the cryostat. Tissues do not need to be fixed. Frozen sections are directly mounted onto microscope slides.
Components/Accessories:
Capacity: Bone samples and other tissues that are difficult to mount as frozen sections are processed with the CryoJane. The unit provides a permanent adhesive mounting onto slides.
Components/Accessories:
Capacity: Slide mounted specimens can be cover slipped and preserved in mounting medium at this station that provides -air filtration to remove fumes. Small batches of slide mounted specimens can be stained at this station.
Components/Accessories:
Capacity: Microscope slides are examined and photographed at this station. Photomicrographs are saved onto CDs for permanent storage and publication.
| Instruments | Charge/Slide | Charge/Year with Unlimited Access | ||
| School of Pharmacy | Other USC Users | School of Pharmacy | Other USC Users | |
| All instruments | $10 | $10 | $3,000 | $3,000 |
Special rates:
Investigators who intend to process many tissue samples and produce many microscope slides are encouraged to talk to Professor Adams about bulk rates. Investigators who pay the bulk rate can be trained to process tissues and will be given access to the lab and its resources.
Depending on the volume of tissues and slides, samples are usually produced in less than a week.
The USC School of Pharmacy Histology Core is available to all investigators for tissue processing, slide preparation, staining and photomicroscopy. Experts are available to advise on immunohistochemical staining and other special staining procedures. Experts are also available to consult on the assessment of the histology and pathology of microscopic specimens.
1985 Zonal Avenue, PSC 402
Los Angeles, CA 90033
James Adams. PhD
Associate Professor
Telephone: 323-442-1362
Fax: 323-442-1681
E-mail: jadams@usc.edu
Lina He, PhD
Histology Core Associate
Telephone: 323-442-3119
Email address: linahe@usc.edu
Lina He, M.D.
Department of Pharmacology and Pharmaceutical Sciences
USC School of Pharmacy, PSC 402B
Telephone: (323) 442-2157
Email: linahe@usc.edu