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Established in 2000 to provide state-of-the-art equipment in microscopy and digital imaging to investigators at CHLA. Core personnel perform microscopy and image analysis on research samples and also provide ongoing training and education in the use of the imaging instruments and software, provides consultation regarding experimental approach, recent literature, new methods, and assists with grant and manuscript preparation. Since its opening in March 2000, the Image Core has been cited in more than 35 peer-reviewed Childrens Hospital Los Angeles publications.
Training in the use of microscopes and digital image acquisition
Training and assistance in digital image analysis
Consultation regarding experimental approach and digital publication
Provision of workshops in cellular imaging and Web-based tutorials
Equipped for a variety of visible wavelengths (UV to far red)
Differential interference contrast (DIC) optics
SKY Spectral Imager
Hamamatsu CCD Camera
HP6200 PC (Software: Metamorph, Photoshop, Office)
Slide scanner
Flatbed scanner
Color printer
Lasers provide excitation at 405, 458, 488, 514, 561 & 633 nm.
34 Spectral detection channels
Axio Observer Z1 Inverted microscope with 3-axis motorized stage equipped with high N.A. objectives and DIC optics
Zeiss Axioplan Microscope
SPOT QE Color Digital Camera
3x5 Large Format Film Camera
Contact us for further information
The primary focus of the DCI Core is technology transfer and provision of facilities for acquisition and analysis of histological and cytological preparations. An important aspect of this technology transfer is to provide training in the use of digital imaging devices, as well as in the application of image analysis procedures for generation of quantitative data.
Childrens Hospital Los Angeles
Saban Research Institute Building Rooms 435 and 338
Smith Research Tower Room 1030
Richard Simerly, PhD
Director
Telephone: (323) 361-8608
Fax: (323) 361-2019
Email: rsimerly@chla.usc.edu
Yannan Ouyang, PhD
Supervisor
Telephone: (323) 361-2548
Fax: (323) 361-7921
Email: youyang@chla.usc.edu
Contact Dr. Yannan Ouyant at
(323) 361-2548 or via e-mail
at youyang@chla.usc.edu